Influence of different separating mediums applied on the immediate dentin sealing in the tensile strength of temporary restorations / Influência de diferentes agentes isolantes aplicados sobre o selamento dentinário imediato na resistência à tração de restaurações provisórias

Objective: To evaluate the ability of different separating mediums to prevent adhesion between provisional restorations and hybridized dentin surfaces when used with the Immediate Dentin Sealing technique (IDS). Material and Methods: 120 extracted human teeth ­ 40 premolars and 80 molars ­ were selected to integrate acrylics samples separated into 4 different groups (n=10) according to the separating medium used for IDS protection. Forty samples were made of three teeth mounted in a self-cure acrylic resin block (Jet, Classic, São Paulo, Brazil) arranged side by side, simulating the positioning and proximal contacts present in the dental arch between the second premolar, first molar and second molar. A standard dental preparation for ultra-thin occlusal veneer was performed and complete occlusal dentin exposure was achieved by selective removal of the occlusal enamel with a high-speed conical round burr (Code: 1801.4138 FG, KG Sorensen, Medical Burs Ind. e Com. de Pontas e Brocas Cirúrgicas Ltda, São Paulo, Brazil). Dentin hybridization were performed on the control group and 3 different groups of separating mediums (n=10): PC­commercial provisional separating agent (Pro-V Coat, Bisco, Schaumburg, USA); GG­glycerin gel (K-Y Gel; Johnson & Johnson Industry and Commerce LTDA, São Paulo, Brazil); PJ­petroleum jelly (Rioquímica, São Paulo, Brazil). Provisionalization were manufactured on the prepared teeth. After 2 weeks, the tensile test was performed, and the analysis of the failure pattern was performed by 3D laser confocal microscopy and Scanning Electron Microscopy (SEM). The data were analyzed with the 1-way ANOVA and the Tukey test (α = 0.05). Results: Significantly lower tensile strength values were found for the commercial provisional separating agent (30.39 ± 10.01 N) compared to others (p < 0.05). Conclusion: The commercial provisional separating agent showed greater effectiveness on protecting hybridized dentin against the provisional restorations (AU)

Objetivo: Avaliar a capacidade de diferentes agentes isolantes em prevenir a adesão entre as restaurações provisórias e a superfícies de dentina hibridizadas quando usados com a técnica do Selamento Dentinário Imediato (IDS). Material e Métodos: 120 dentes humanos extraídos - 40 pré-molares e 80 molares - foram selecionados para integrar as amostras de acrílico divididas em 4 grupos diferentes (n = 10) de acordo com o meio de separação usado para proteção IDS. Foram confeccionadas 40 amostras de três dentes montados em um bloco de resina acrílica autopolimerizável (Jet, Classic, São Paulo, Brasil) dispostos lado a lado, simulando o posicionamento e os contatos proximais presentes na arcada dentária entre o segundo pré-molar, primeiro molar e segundo molar. Um preparo dentário padrão para laminado oclusal ultrafino foi realizado e a exposição completa da dentina oclusal foi obtida pela remoção seletiva do esmalte oclusal com uma broca cônica arredondada de alta rotação (Código: 1801.4138 FG, KG Sorensen, Medical Burs Ind. E Com. de Pontas e Brocas Cirúrgicas Ltda, São Paulo, Brasil). A hibridização da dentina foi realizada no grupo controle e em 3 grupos diferentes de agentes isolantes (n = 10): PC - agente de separação provisório comercial (Pro-V Coat, Bisco, Schaumburg, EUA); GG ­ gel de glicerina (K-Y Gel; Johnson & Johnson Industry and Commerce LTDA, São Paulo, Brasil); PJ ­ vaselina (Rioquímica, São Paulo, Brasil). As restaurações provisórias foram fabricadas nos dentes preparados. Após 2 semanas, foi realizado o teste de tração, e a análise do padrão de falha foi realizada por microscopia confocal a laser 3D e Microscopia Eletrônica de Varredura (MEV). Os dados foram analisados com a ANOVA de 1 fator e o teste de Tukey (α = 0,05). Resultados: Valores de resistência à tração significativamente menores foram encontrados para o agente separador provisório comercial (30,39 ± 10,01 N) em comparação com os demais (p < 0,05). Conclusão: O agente separador provisório comercial mostrou maior eficácia para a proteção da dentina hibridizada contra as restaurações provisórias (AU)

Effects of different types of dentin protective membranes on dentin adhesion properties / 西安交通大学学报(医学版)

Objective: To investigate the effects of three different types of dentin protective membranes (HyC, SBU and S3) on dentin adhesive properties. Methods: Fresh isolated molar teeth were collected, and smooth dentin surface was obtained after embedding, cutting and polishing. In the control group, dentin protective membrane was not applied, while the remaining three groups were coated with three different types of dentin protective membranes, and the preformed resin prosthesis was cemented with resin cement. A strip dentin-resin sample with a cross-sectional area of 0.9 mm×0.9 mm was obtained by a precision cutting machine. The tensile force was tested at the loading speed of 0.5 mm/min with a universal testing machine until the dentin-resin strip was broken. The maximum tensile force during the loading process was automatically recorded by the computer, and the microstructure and fracture mode of the section were observed by a scanning electron microscope. Results: After coating dentin protective membranes, the tensile strength of each experimental group was significantly increased compared with that of the control group (P<0.05), and the tensile strength of group HyC was significantly smaller than that of group SBU and group S3 (P<0.05). The tensile fracture mode shown by microstructure observation was mainly interfacial failure. Conclusion: Different types of dentin protective membranes can increase the adhesive properties between dentin and resin prosthesis. SBU and S3 can improve the adhesion strength more significantly than HyC.

Effect of temporary cements on the bond strength of a resin cement to a pre-hybridized dentin / Influência de cimentos temporários na resistência de união de um cimento resinoso sobre a dentina pré-hibridizada

This study assessed the bond strength of a resin cement when Immediate Sealed Dentin (ISD) was contaminated by different temporary cements applying a microshear test. Forty-eight fragments of third molar crowns were mounted in acrylic resin and worn until the dentin was exposed. The substrates were sealed with a 3-step conventional adhesive system and divided into four groups (n=12): Control (without temporary cement) and three groups that used different temporary cements - Provy, RelyX Temp NE, and Provitemp. The temporary cements were removed after 7 days of storage. Resin cement RelyX ARC was applied to the contaminated substrates. Microshear bond strength was evaluated using a universal testing machine (0.5 mm/min). Data was analyzed with ANOVA and a Dunnett post-hoc test (p<0.05). Additionally, three samples from each group were prepared for scanning electron microscope analysis. Means and standard deviations of microshear bond strength (MPa) were 17.96±1.65, 16.70±1.43, 20.43±1.30 and 33.75±4.72 for Control, Provy, RelyX Temp, and Provitemp, respectively. Statistically significant difference was observed between the Control and Provitemp samples (p=0.0002). Adhesive failures were dominant (50%), followed by cohesive failures with cement (26%), and cohesive failures with dentin (24%). Residues of Provy and Provitemp were observed on the sealed dentin. Provitemp temporary cement sealing increased the microshear bond strength of the resin cement on dentin treated with IDS.

Este estudo investigou a influência de cimentos temporários na resistência de união de um cimento resinoso em dentina selada imediatamente. Quarenta e oito fragmentos obtidos das coroas de terceiros molares foram incluídos em tubos de PVC com resina acrílica e desgastados até exposição da dentina. Os substratos foram tratados com adesivo convencional de três passos e divididos em quatro grupos: Controle (sem cimento temporário) e três grupos usando diferentes cimentos temporários ­ Provy, RelyX Temp NE e Provitemp. Após sete dias, os cimentos foram removidos e o cimento RelyX ARC aplicado sobre o substrato contaminado. A resistência de união foi avaliada em uma máquina de ensaio universal (0,5 mm/min). Os dados foram analisados pelos testes estatísticos ANOVA e Dunnet (p<0,05). Três amostras de cada grupo foram preparadas para análise em micróscopio eletrônico de varredura. Os valores médios e desvio padrão da resistência de união foram 17,96 ±1,65, 16,70 ±1,43, 20,43±1,30 e 33,75 ±4,72 para Controle, Provy, RelyX Temp e Provitemp, respectivamente. Diferença significativa foi observada entre os grupos Controle e Provitemp (p=0,0002). Com relação ao padrão de fratura, a maioria das falhas foram adesivas (50%), seguida por coesiva em cimento (26%) e coesiva em dentina (24%). Resíduos dos cimentos Provy e Provitemp foram observados na dentina selada. Cimento temporário Provitemp aumentou a resistência de união por microcisalhamento do cimento resinoso a dentina com IDS.

The effect of IDS (immediate dentin sealing) on dentin bond strength under various thermocycling periods

PURPOSE: The purpose of this study was to find out the effect of immediate dentin sealing (IDS) on bond strength of ceramic restoration under various thermocycling periods with DBA (dentin bonding agent system). MATERIALS AND METHODS: Fifty freshly extracted human mandibular third molars were divided into 5 groups (1 control and 4 experimental groups) of 10 teeth. We removed enamel layer of sound teeth and embedded them which will proceed to be IDS, using All Bond II. A thermocycling was applied to experimental groups for 1, 2, 7, 14 days respectively and was not applied to control group. IPS Empress II for ceramic was acid-etched with ceramic etchant (9.5% HF) and silane was applied. Each ceramic disc was bonded to specimens with Duo-link, dual curable resin cement by means of light curing for 100 seconds. After the cementation procedures, shear bond strength measurement and SEM analysis of the fractured surface were done. The data were analyzed with a one-way ANOVA and Tukey multiple comparison test (alpha=.05). RESULTS: There were no statistically significant differences between 4 experimental groups and control group, however the mean value started to decrease in group 7d, and group 14d showed the lowest mean bond strength in all groups. Also, group 7d and 14d showed distinct exposed dentin and collapsed hybrid layer was observed in SEM analysis. CONCLUSION: In the present study, it can be concluded that ceramic restorations like a laminate veneer restoration should be bonded using resin cement within one week after IDS procedure.

Microtensile bond strength of resin inlay bonded to dentin treated with various temporary filling materials / 대한치과보존학회지

OBJECTIVES: This study was aimed to determine the effects of temporary sealing materials on microtensile bond strength between resin-coated dentin and resin inlay and to compare the bonding effectiveness of delayed dentin sealing and that of immediate dentin sealing. MATERIALS AND METHODS: The teeth were divided into 4 groups: group 1, specimens were prepared using delayed dentin sealing after temporary sealing with zinc oxide eugenol (ZOE); group 2, specimens were prepared using immediate dentin sealing and ZOE sealing; group 3, specimens were prepared using immediate dentin sealing and Dycal (Dentsply) sealing; group 4, specimens were prepared using immediately sealed, and then temporarily sealed with a resin-based temporary sealing material. After removing the temporary sealing material, we applied resin adhesive and light-cured. Then the resin inlays were applied and bonded to the cavity with a resin-based cement. The microtensile bond strength of the sectioned specimens were measured with a micro-tensile tester (Bisco Inc.). Significance between the specimen groups were tested by means of one-way ANOVA and multiple Duncan's test. RESULTS: Group 1 showed the lowest bond strength, and group 4 showed the highest bond strength (p < 0.01). When temporary sealing was performed with ZOE, immediate dentin sealing showed a higher bonding strength than delayed dentin sealing (p < 0.01). CONCLUSIONS: Based on these results, immediate dentin sealing is more recommended than delayed dentin sealing in bonding a resin inlay to dentin. Also, resin-based temporary sealing materials have shown the best result.

Influence of air abrasion and different dentin sealing techniques on microtensile bond strength to dentin / 대한치과보철학회지

PURPOSE: The purpose of this study was to evaluate the effect of various methods of dentin bonding agent application and air abrasion pretreatment on microtensile bond strength between dentin and resin, using a self-etching adhesive system. MATERIAL AND METHODS: Thirty freshly extracted human molars were obtained and divided into 6 groups of 5 teeth. A 2-step self etching adhesive system (Clearfil SE Bond) was used for all groups. The control specimens were prepared using a direct immediate bonding technique. The delayed dentin sealing specimens were prepared using an indirect approach without dentin prebonding. The immediate dentin sealing specimens were prepared using dentin prebonding immediately following preparation. Immediate dentin sealing teeth and delayed dentin sealing teeth had provisional restorations using Fermit for two weeks. Then all specimens of each group were divided into two groups of three, depending on air abrasion pretreatment. Composite "crowns" were incrementally built on and specimens were stored in water for 24 hours. All teeth were prepared for a microtensile bond strength test. Bond strength data were analyzed with a one-way ANOVA test, and post hoc comparison was done using the Scheffe's test. RESULTS: The mean microtensile bond strengths of all groups were not statistically different from each other. CONCLUSION: When preparing teeth for indirect restorations, IDS and DDS with Clearfil SE bond, have no difference on the microtensile bond strength between dentin and resin. Air abrasion pretreatment did not affect the microtensile bond strength when using IDS and DDS with Clearfil SE bond.

An effect of immediate dentin sealing on the shear bond strength of resin cement to porcelain restoration

PURPOSE: The aim of this study was to determine differences in shear bond strength to human dentin using immediate dentin sealing (IDS) technique compared to delayed dentin sealing (DDS). MATERIAL AND METHODS: Forty extracted human molars were divided into 4 groups with 10 teeth each. The control group was light-cured after application of dentin bonding agent (Excite(R) DSC) and cemented with Variolink(R) II resin cement. IDS/SE (immediate dentin sealing, Clearfil(TM) SE Bond) and IDS/SB (immediate dentin sealing, Adapter(TM) Single Bond 2) were light-cured after application of dentin bonding agent (Clearfil(TM) SE Bond and Adapter(TM) Sing Bond 2, respectively), whereas DDS specimens were not treated with any dentin bonding agent. Specimens were cemented with Variolink(R) II resin cement. Dentin bonding agent (Excite(R) DSC) was left unpolymerized until the application of porcelain restoration. Shear strength was measured using a universal testing machine at a speed of 5 mm/min and evaluated of fracture using an optical microscope. RESULTS: The mean shear bond strengths of control group and IDS/SE group were not statistically different from another at 14.86 and 11.18 MPa. Bond strength of IDS/SE group had a significantly higher mean than DDS group (3.14 MPa) (P < .05). There were no significance in the mean shear bond strength between IDS/SB (4.11 MPa) and DDS group. Evaluation of failure patterns indicates that most failures in the control group and IDS/SE groups were mixed, whereas failures in the DDS were interfacial. CONCLUSION: When preparing teeth for indirect ceramic restoration, IDS with Clearfil(TM) SE Bond results in improved shear bond strength compared with DDS.

The effect of various bonding systems on the microtensile bond strength of immediate and delayed dentin sealing / 대한치과보존학회지

The purpose of this study was to compare the effect of various dentin bonding systems on microtensile bond strength of immediate dentin sealing (IDS) and delayed dentin sealing (DDS). Eighteen extracted permanent molars were used in this study. The teeth for DDS group were restored with a provisional restorations, and immersed in saline solution for 1 week, and divided into 3 subgroups according to various dentin bonding adhesives; SB subgroup (3 step total-etch adhesive), SE subgroup (2 step self-etch adhesive), XE subgroup (1 step self-etch adhesive). In IDS group, the teeth were divided into 3 subgroups, and applied with bonding adhesives as in DDS group. The teeth were restored with provisional restorations, and immersed in saline solution for 1 week. Indirect composite disc was cemented with resin cement, and all specimens were subjected to microtensile bond strength. The data were statistically analyzed with one-way ANOVA and Student t-test. The results were as follows: 1. The IDS group showed significantly higher microTBS than DDS group in 3 step total-etch and 2 step self-etch adhesive (p < 0.05). 2. In IDS and DDS group, 3 step total-etch adhesive showed the highest microTBS value, followed by 2 step self-etch, and 1 step self-etch adhesive. In IDS group, the microTBS value for 1 step self-etch adhesive was significantly different from those of the other subgroups (p < 0.05), and in DDS group, there were statistical differences in all subgroup (p < 0.05). 3. Failure modes of tested dentin bonding adhesives were mostly mixed failure and only 1 step self-etch adhesive showed adhesive failure.

The comparison of microtensile bond strength with immediate and delayed dentin sealing / 대한치과보철학회지

PURPOSE: The purpose of this study was to evaluate the effect of various methods of dentin bonding agent application on microtensile bond strength between dentin and resin, using a 2-step etch-and-rinse dentin bonding agent. MATERIAL AND METHODS: Twenty freshly extracted human molars were obtained and divided into 4 groups of 5 teeth. 2-step etch-and-rinse dentin bonding agent was used for all groups. The control specimens were prepared using a direct immediate bonding technique. The delayed dentin sealing (A, C) specimens were prepared using an indirect approach with delayed dentin sealing. For group A, resin was built-up on uncured dentin bonding agent, and for group C, resin was built-up on pre-cured dentin bonding agent. Preparation of the immediate dentin sealing (B) specimens also used an indirect approach with immediate dentin sealing immediately following preparation. All teeth were prepared for a microtensile bond strength test. Specimens were stored in water for 24 hours. Ten beams (1.0 x 1.0 x 11 mm) from each tooth were selected for testing. Bond strength data (MPa) were analyzed with a one-way ANOVA test, and post hoc comparison was done using the Scheffe's test. RESULTS: The mean microtensile bond strengths of control group, B and C (DDS with pre-cure) were not statisticaaly different from each other at 32.7, 33.3, 34.2 MPa. the bond strength for group A (DDS without pre-cure), 19.5 MPa, was statistically different (P < .01) from the other 3 groups. CONCLUSION: When preparing teeth for indirect bonded restorations, DDS with pre-curing dentin bonding agent and IDS results in the same bond strength between dentin and resin. On the contrast, the bond strength was decreased when DDS without pre-curing dentin bonding agent was used.